Microglial Repopulation Reverses Age-Related Sleep Decline

Published on October 3, 2022

Imagine your body as a finely-tuned machine, and one of the key parts responsible for maintaining its smooth operation is the brain. Just like any other machine, as it ages, certain parts may start to wear out or function less efficiently. Well, researchers have discovered that in the case of sleep-wake cycles, microglia, a type of cells in the brain, play a vital role. These microglia help regulate the balance between sleep and wakefulness. In aging mice, scientists observed a decline in stable wakefulness at nighttime. Furthermore, they noticed that microglial processes in the brain regions responsible for sleep regulation became shorter as the mice aged. However, when the microglia were depleted and then repopulated in the adult brain, the decline in stable wakefulness was reversed. This finding suggests a strong connection between aged microglia and the deterioration of stable wakefulness in older brains. If you’re interested in learning more about this fascinating research on sleep and aging, check out the full article!

Changes in wake/sleep architecture have been observed in both aged human and animal models, presumably due to various functional decay throughout the aging body particularly in the brain. Microglia have emerged as a modulator for wake/sleep architecture in the adult brain, and displayed distinct morphology and activity in the aging brain. However, the link between microglia and age-related wake/sleep changes remains elusive. In this study, we systematically examined the brain vigilance and microglia morphology in aging mice (3, 6, 12, and 18 months old), and determined how microglia affect the aging-related wake/sleep alterations in mice. We found that from young adult to aged mice there was a clear decline in stable wakefulness at nighttime, and a decrease of microglial processes length in various brain regions involved in wake/sleep regulation. The decreased stable wakefulness can be restored following the time course of microglia depletion and repopulation in the adult brain. Microglia repopulation in the aging brain restored age-related decline in stable wakefulness. Taken together, our findings suggest a link between aged microglia and deteriorated stable wakefulness in aged brains.

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