Unlocking the Secrets of Glioma Stem Cells and circRNAs

Published on April 13, 2022

In this exciting study, scientists delved into the world of glioma stem cells to investigate how a particular circular RNA (circ-ASB3) controls the progression of glioma, a type of brain tumor. Using cutting-edge techniques like biological information analysis and RNA transfection, they uncovered a regulatory pathway involving circ-ASB3, miR-543, and Twist1. It turns out that circ-ASB3 can bind to miR-543, reducing its expression and unleashing the inhibitory effect on Twist1. The result? Increased proliferation, invasion, and migration of glioma cells in vitro, along with suppressed apoptosis and induced cell cycle arrest. These findings shed light on potential biomarkers and therapeutic targets for glioma patients, offering hope for improved treatments in the future.

ObjectiveIn our research we try to explore whether glioma stem cell containing circRNAs signal pathway could regulate glioma malignant progression and elaborate its possible mechanism.MethodsIn this study, we used biological information analysis to build an RNA regulatory network and then proceeded RT-PCR to screen target RNAs, after that we clarified the targeting relationship between circRNA-miRNA-mRNA through double luciferase gene assay, RNA pull down experiment, PCR and Western Blot. Finally we adopted RNA transfection to identify its impact on glioma cell proliferation, invasion, migration, apoptosis and cell cycle.Resultscirc-ASB3 was significantly up-regulated in glioma stem cells compared with glioma cells. The circ-ASB3/miR-543/Twist1 axis was discovered to be a possible regulatory pathway in glioma, circ-ASB3 could adsorb and targeted bind to miR-543, down-regulate miR-543 expression, thus release its targeted inhibition to Twist1. Circ-ASB3 was shown to increase glioma cell proliferation, invasion, and migration in vitro via miR-543/Twist1 axis. Meanwhile glioma cell apoptosis could be inhibited, and cell cycle arrest could be induced through this signaling pathway.Conclusioncirc-ASB3 could enhance glioma malignancy via miR-543/Twist1 axis, resulting in the discovery of new biomarkers and possible therapeutic targets for these patients.

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