Triple Transgenic Mice Show Altered Memory, Synaptic Plasticity, and Circadian Rhythm in Alzheimer’s Model

Published on March 31, 2022

Imagine a clock that keeps getting slower and slower, throwing off the timing of everything around it. This is similar to what happens in Alzheimer’s disease (AD). AD is like a glitchy clock that affects not only memory and brain function, but also the body’s internal rhythm called the circadian rhythm. In this study with mice, scientists compared normal mice to triple transgenic mice that mimic AD symptoms. They found that the AD mice had disrupted circadian variations in memory performance, synaptic plasticity (which is how neurons communicate), and mitochondrial function (the powerhouse of cells). These three important aspects were all out of sync, just like a trio of clocks ticking at different speeds. It’s as if the clocks controlling memory, communication between neurons, and energy production were all malfunctioning in the AD mice. This research sheds light on how circadian misalignment can affect multiple dimensions of brain health in Alzheimer’s disease.

Alzheimer’s disease (AD) is characterized by progressive memory deficits accompanied by synaptic and metabolic deficits, namely of mitochondrial function. AD patients also display a disrupted circadian pattern. Thus, we now compared memory performance, synaptic plasticity, and mitochondria function in 24-week-old non-transgenic (non-Tg) and triple transgenic male mice modeling AD (3xTg-AD) at Zeitgeber 04 (ZT-4, inactive phase) and ZT-16 (active phase). Using the Morris water maze test to minimize the influence of circadian-associated locomotor activity, we observed a circadian variation in hippocampus-dependent learning performance in non-Tg mice, which was impaired in 3xTg-AD mice. 3xTg-AD mice also displayed a lack of circadian variation of their performance in the reversal spatial learning task. Additionally, the amplitude of hippocampal long-term potentiation also exhibited a circadian profile in non-Tg mice, which was not observed in 3xTg-AD mice. Moreover, cerebral cortical synaptosomes of non-Tg mice also displayed a circadian variation of FCCP-stimulated oxygen consumption as well as in mitochondrial calcium retention that were blunted in 3xTg-AD mice. In sum, this multidimensional study shows that the ability to maintain a circadian oscillation in brain behavior, synaptic plasticity, and synaptic mitochondria function are simultaneously impaired in 3xTg-AD mice, highlighting the effects of circadian misalignment in AD.

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