Levodopa and Antibiotic Resistance in Gut Bacteria of PD Rats

Published on February 7, 2023

Just like a master chef creating a new recipe, scientists explored the effects of levodopa treatment on antibiotic resistance in the gut bacteria of rats with Parkinson’s disease (PD). Levodopa, the backbone of PD treatment, has been known to cause side effects. However, this study sought to investigate if levodopa could potentially influence antibiotic resistance in the gut. Through metagenomic sequencing, they found that levodopa altered the composition of gut bacteria and changed the biomarkers associated with them. Interestingly, when higher doses of levodopa were administered, specific antibiotic resistance genes decreased in abundance. This suggests that combining levodopa with certain antibiotics may minimize antibiotic resistance in gut bacteria. These findings provide a foundation for future research on treating PD with a combination of levodopa and targeted antibiotics.

The second most prevalent neurodegenerative ailment, Parkinson’s disease (PD), is characterized by both motor and non-motor symptoms. Levodopa is the backbone of treatment for PD at the moment. However, levodopa-induced side effects, such as dyskinesia, are commonly seen in PD patients. Recently, several antibiotics were found to present neuroprotective properties against neurodegenerative and neuro-inflammatory processes, which might be developed to effective therapies against PD. In this study, we aimed to identify if levodopa treatment could influence the gut bacterial antibiotic resistance in PD rat. Fecal samples were collected from healthy rats and 6-OHDA induced PD rats treated with different doses of levodopa, metagenomic sequencing data showed that levodopa resulted in gut bacteria composition change, the biomarkers of gut bacteria analyzed by LEfSe changed as well. More interestingly, compared with levodopa (5 mg/kg)-treated or no levodopa-treated PD rats, levodopa (10 mg/kg) caused a significant decrease in the abundance of tetW and vanTG genes in intestinal bacteria, which were related to tetracycline and vancomycin resistance, while the abundance of AAC6-lb-Suzhou gene increased apparently, which was related to aminoglycosides resistance, even though the total quantity of Antibiotic Resistance Gene (ARG) and Antibiotic Resistance Ontology (ARO) among all groups did not significantly differ. Consequently, our results imply that the combination of levodopa and antibiotics, such as tetracycline and vancomycin, in the treatment of PD may decrease the amount of corresponding antibiotic resistance genes in gut bacteria, which would give a theoretical basis for treating PD with levodopa combined with tetracycline and vancomycin in the future.

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