Just like a superhero team-up, probiotics from tempeh join forces with the Gut-Brain-Axis to improve cognitive function in elderly individuals. In a study involving 93 subjects, probiotic supplementation derived from tempeh was provided for 12 weeks. The results showed that the probiotics, identified as L. fermentum A2.8, enhanced memory, learning process, language, and visuospatial function. Interestingly, a higher concentration of probiotics at 108 CFU/mL was found to be more effective in improving the learning process. The presence of genes encoding glutamate decarboxylase (gad) was detected in the probiotic isolate, which plays a crucial role in synthesizing a neurotransmitter called gamma-aminobutyric acid (GABA). GABA is essential for cognitive function, acting like a conductor who helps orchestrate brain activity. This research opens doors to potential interventions for cognitive impairment through the consumption of tempeh or other probiotic-rich foods. If you’re curious to learn more about how probiotics can boost brain power, check out the full article linked below!

IntroductionOral consumption of probiotics can alter Gut Microbiota by causing changes in the production of probiotic derivatives. Therefore, by utilizing Gut-Brain-Axis (GBA), probiotics could provide an opportunity for central nervous system (CNS) modulation, including cognitive function. Tempeh is a traditional Indonesian food rich in probiotics and beneficial for cognitive function. However, the type of probiotics that play a role in cognitive improvement and the number of probiotics needed for the benefits of increasing cognitive function was unknown.MethodThis experimental study involved a total of 93 subjects, divided into 3 groups: A, B and C/control (n: 33, 32, and 28), who were provided with probiotic supplementation isolated from tempeh for 12 weeks intervention. Inclusion criteria were age > 60 years, and memory impairment with the third repetition value of Word List Memory Immediate Recall (WLMIR) < 7. Subjects with diabetes were excluded. Cognitive function examinations were carried out before and after treatment. The tempeh-derived probiotics were prepared trough several processes. Genomic isolation, detection of GABA-encoding genes, and species identification using the 16S-rRNA gene encoding were performed.ResultsThe probiotics isolate used in the intervention was identified as Limosilactobacillus fermentum. We assigned this isolate as L. fermentum A2.8. The presence of the gene encoding GABA was found on this isolate. There was an increase in the cognitive domains of memory, learning process, and verbal fluency (p < 0.05) in group A (probiotics at concentration of 108 CFU/mL). Memory function, visuospatial, and verbal fluency improved (p < 0.05) in group B (probiotics at concentration of 107 CFU/mL). Only an increase in the memory domain was observed in the control group. Improvement of the learning process occurred only in group A (p = 0.006).ConclusionAdministration of probiotics derived from L. fermentum A2.8 increased the cognitive domains of memory, language and visuospatial function. However, probiotic supplementation at a concentration of 108 CFU/mL was better in improving the learning process. This study succeeded in detecting Lactic Acid Bacterial isolates L. fermentum A2.8 that enclosed gene encoding glutamate decarboxylase (gad) which is involved in the synthesis of -aminobutyric acid (GABA), a neurotransmitter vital for cognitive function.

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